Carcinogenic dyes are still used by many manufacturers due to their being cheap, despite the fact that European laws prohibit their use. These dyes can cause a variety of adverse health effects, carcinogenic or otherwise, as well as allergic reactions when brought into contact with the skin.
Allergenic dyes have considerable sensitising potential.
Their use in products intended to come into contact with the skin must be avoided in order to safeguard children against the onset of allergic conditions such as, for example, dermatitis, itching and redness.

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    Their use in products intended to come into contact with the skin must be avoided in order to safeguard children against the onset of allergic conditions such as, for example, dermatitis, itching and redness.
    Colorants are one of the most important groups of substances used in the clothing industry.
    On account of their properties the molecules of some colorants can be absorbed by the skin.
    In this case the presence of cancerogenous and allergenic colorants may be harmful to the health of the consumer.
    Since the colorants are used on articles of clothing intended for prolonged contact with the skin, the release of the eco safe mark is subject to analysis of certain cancerogenous and allergenic colorants in order to limit theexposure of consumers to these substances and consequently also the risks connected with their properties.
    The test method used to examine colorants is based on extraction of the colorants (from the material or leather) with a solvent.
    This analysis is aimed at extracting all the colorants present from the sample analyzed.
    On obtaining the extract the laboratory determines the presence of the colorants in question using chromatographic analysis.
    The analysis has the purpose of determining the presence of certain colorants in order to ascertain whether they are present in quantities below the levels allowed for release of the eco safe mark.

Phthalates are organic chemicals produced from petroleum and are among the most common plasticisers in the world.
Their purpose is to soften plastics. In clothing, they are generally used to produce T-shirt prints and other soft plastic elements. Many phthalates are classified as toxic to reproduction and in some cases are suspected of being responsible for adverse health effects, such as allergies and liver and kidney damage.

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    Phthalates are a group of substances used in the textile industry mainly for the production of soft plastic articles prints and coatings.
    The presence in toys and articles used in early childhood care of certain phthalates has been limited by an EU directive for a long time.
    The reasoning behind the EU decision to introduce this limitation is connected with the toxicity of some phthalates and the fact that children, especially the very young, have the habit of putting objects that they find in their mouths.
    This behavior makes them particularly exposed to the introduction of these substances into their bodies if they are present in the products intended for them.
    Since small children have this habit with all the objects surrounding them, and since even older children may be exposed to these substances through contact with the skin and the mouth, clothing may also represent a source of exposure to these substances.
    Therefore, to obtain the eco safe mark, the presence of certain phthalates probably contained in the materials of all articles of clothing intended for children is determined.
    The test method used involves extraction of the substances present in the sample of soft plastic parts or coatings.
    The sample thus obtained is then analyzed by gas chromatography.
    The purpose of the analysis is the ascertain the presence of one or more of the phthalates selected in order to determine whether the quantity present is below the level allowed for use of the eco safe mark.

Heavy metals include lead, often used for buttons and plastic elements, nickel, used for the metal finish of belt buckles and so on, and chromium, often found in leather, as well as cadmium, mercury, arsenic and other metals present as residues of the industrial processes undergone by clothing.
Heavy metals can have diverse adverse health effects, depending on the type of metal, and can be carcinogenic, can affect the central nervous system, can be sensitising, or can cause irritation of the skin. Many metals are also potentially dangerous for the environment.

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    Heavy metals may be present in articles of clothing as the residues of production processes (coloring for example).
    These elements may cause numerous effects harmful to the consumers' health (from allergic reactions to cancerogenous effects) and the environment.
    Consumers, and especially children, may be exposed to the metals contained in articles of clothing both orally (putting products in their mouths) or by skin contact (during normal use of clothing).
    Given the highly toxic properties of certain metals and their possible presence due to numerous sources of contamination, release of the eco safe mark is subject to the observance of certain concentration limits of these metals. Concentrations are determined, depending on the case, as total content or as an extraction.
    Determination of the total content is connected with an evaluation that also takes into account implications for the disposal of the finished product and therefore its impact on the environment.
    The test methods used for determining the content of heavy metals are of two types:
    - methods for determining the total content of heavy metals
    - methods for determining the release of heavy metals from the materials tested
    In the first case the method requires the dissolution of the sample in order to determine the total content of heavy metals in the part tested.
    In the second case, the method requires the extraction of the sample using a solution that simulates the effect of sweat and successive analysis of the solution obtained.
    In this way the method determines the concentration ofheavy metals which can be extracted from the material in question during the foreseeable use of the product by the consumer.

Formaldehyde is a volatile organic compound mainly used for the production of resins, and is sometimes used in the textile industry to make fabrics crease resistant. Being a powerful bactericide, formaldehyde is also used to treat fabrics for conservation purposes.
Formaldehyde can cause irritation of the skin and allergic reactions, and is suspected of being carcinogenic.

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    Phthalates are a group of substances used in the textile industry mainly for the production of soft plastic articles prints and coatings.
    The presence in toys and articles used in early childhood care of certain phthalates has been limited by an EU directive for a long time.
    The reasoning behind the EU decision to introduce this limitation is connected with the toxicity of some phthalates and the fact that children, especially the very young, have the habit of putting objects that they find in their mouths.
    This behavior makes them particularly exposed to the introduction of these substances into their bodies if they are present in the products intended for them.
    Since small children have this habit with all the objects surrounding them, and since even older children may be exposed to these substances through contact with the skin and the mouth, clothing may also represent a source of exposure to these substances.
    Therefore, to obtain the eco safe mark, the presence of certain phthalates probably contained in the materials of all articles of clothing intended for children is determined.
    The test method used involves extraction of the substances present in the sample of soft plastic parts or coatings.
    The sample thus obtained is then analyzed by gas chromatography.
    The purpose of the analysis is the ascertain the presence of one or more of the phthalates selected in order to determine whether the quantity present is below the level allowed for use of the eco safe mark.

Particular attention is also paid to small parts such as sequins.
In order to simulate the force that the child may exercise with teeth and fingers children's clothing are tested to verify the force needed to separate small parts from clothing.
When small parts are not graspable the clothing articles are washed in a special equipment and after the test, the small parts should remain not graspable.
The scope of the verification is to assure small parts are not accessible to the children.

Attention to hazardous drawstrings. The release of eco safe mark is dependent upon the verification of children's clothing with the scope to verify that the items have the correct design and construction. The scope of the verification is to underline the conformity for the use of the eco safe mark.